Determination of antixidant capacity in Pluchea indica Less leaves extract and its frantions

Widyawati, Paini Sri (2016) Determination of antixidant capacity in Pluchea indica Less leaves extract and its frantions. International Journal of Pharmacy and Pharmaceutical Sciences, 8 (9). pp. 32-36. ISSN 0975-1491

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Abstract

Objective :This study was conducted to determine the DPPH free radical scavenging activity of pluchea extract (PE) and its fraction (ethyl acetate (EAF), n-butanol (BF) and aqueous (AF)) and comparing with the its activity of control antioxidant, such as green tea (GE) and rosemary extracts (RE), α-tocopherol succinate (ATS) and BHT. Methods: The antioxidant activity was evaluated by the decrease in absorbance as the result of DPPH˙ color change from purple to yellow at 517 nm by using a stopped flow spectrometer UV-Vis, AE (Antiradical effeiciency) was a parameter more exactly used to measure the antioxidant power than EC50 (efficient concentration to reduce 50% of DPPH concentration). This parameter was determined by EC50 and TEC50 (the time needed to reach the steady state). Results: PE and its fraction had free radical-scavenging activity compared with control antioxidant. Based on EC50, the orders of ability to scavenging DPPH radicals was determined as the EAF~GE>PE~ATS~ATS~RE>BHT~BF. the results were not exactly because BHT and ATS were potentially as a synthetic antioxidant. Therefore AE parameter was used to determine scavenging activity. The classification order of scavenging activity based on AE was BHT>GE~ATS>EAF>RE~PE~AF. Conclusion : the PE and its fractions had DPPH free radical scavenging activity. based on EC50and AE parameters, the EAF showed the strongest DPPH free radical scavenging activity compared with PE and the other fractions

Item Type: Article
Uncontrolled Keywords: Antioxidant activity, 2.2-diphenyl-1-picrylhydrazyl (DPPH), pluchea leaves extract, fractions
Subjects: Agriculture and Food Technology
Divisions: Journal Publication
Depositing User: Paini Sri Widyawati
Date Deposited: 24 May 2017 03:46
Last Modified: 14 Jun 2019 08:09
URI: http://repository.wima.ac.id/id/eprint/10682

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