Harlen, Winda Christina, Muchtadi, Tien Ruspriatin and Palupi, Nurheni Sri (2018) β-Carotene bioavailability of palm oil emulsion drink in rats (Rattus norvegicus) blood plasma and liver. International Food Research Journal, 25 (4). pp. 1349-1356. ISSN 19854668 (Print); ISSN 22317546 (Online) (Unpublished)
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Abstract
Palm oil emulsion drink (POED) is a kind of oil-in-water emulsion considered to be a good source of vitamin A as β-carotene but its bioavailability was not well known. The aim of this research were (1) to review the chemical composition and β-carotene content of POED and(2) to evaluate bioavailability of β-carotene based on in vivo study. The chemical composition were determined using proximate analysis, while bioavailability evaluation was carried out by measuring Retinol Accumulation Factor (RAF). Thirty-six male Sprague-dawley rats received a standard diet as an adaptation period and a low-vitamin A-diet as depletion period. In the repletion period, rats were divided into four groups: control (C) group, Positive Control (PC) group, Negative Control (NC) group and Palm Oil Emulsion Drink (POED) group. C group received standard diet, while NC group received depletion diet. The other two groups received depletion diet enriched with pure synthetic β-carotene (for PC group) and POED (for POED group). The POED-enriched-diet and pure-synthetic-β-carotene-diet contained 180 μg β-carotene. At the end of repletion period, all rats were sacrificed then its plasma and liver were colected to provide retinol data. β-carotene content of POED was 211.08 μg/g. After repletion, plasma retinol in POEDgroupincreased up to 1.39 μg/ml in average. Meanwhile, its total liver retinol increased up to 385.76 μg in average. RAF proposed for the test group was 1/9.97, which indicated that 9.97 μg vitamin A from this product was needed to accumulate 1 μg retinol in the liver. Relative bioavailability of POED was 70.91%. It appeared that β-carotene in POED can be well-absorbed by the vitamin A-depleted-rats.
| Item Type: | Article |
|---|---|
| Subjects: | Agriculture and Food Technology |
| Divisions: | Faculty of Agricultural Technology > Food Technology Study Program |
| Depositing User: | Winda Christina Harlen |
| Date Deposited: | 01 Feb 2019 09:00 |
| Last Modified: | 21 Feb 2019 01:38 |
| URI: | http://repository.ukwms.ac.id/id/eprint/16657 |
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