Hypoxic Preconditioning Improved Neuroprotective Effect of Bone Marrow-Mesenchymal Stem Cells Transplantation in Acute Glaucoma Models

Ernawati, Titiek and Suhendro, Gatut and Sudiana, I Ketut and Putra, Suhartono Taat and JM, Harjanto and Sunarjo, - and Turchan, Agus and Rantam, Fedik Abdul (2016) Hypoxic Preconditioning Improved Neuroprotective Effect of Bone Marrow-Mesenchymal Stem Cells Transplantation in Acute Glaucoma Models. Journal of Biomedical Science and Engineering, 9 (4). pp. 245-257. ISSN ISSN Print: 1937-6871, ISSN Online: 1937-688X

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Abstract

This study explored the novel strategy of hypoxic preconditioning of Bone Marrow Mesenchymal Stem Cells (BM-MSCs) before intra vitreal transplantation to improve neuroprotective effects of Retinal Ganglion Cells (RGCs) in Acute Glaucoma Models. The methods of this research were isolated mesenchymal stem cells from the bone marrow of adult wild-type Sprague-Dawley (SD) rats. BM-MSCs were cultured under normoxic or hypoxic (1% oxygen for 24 hours) conditions. Normoxic or hypoxic BM-MSCs were transplanted intravitreally 1 week after ocular hypertension induction by acutely increasing IOP to 100 - 120 mmHg for 60 minutes. Rats were killed 4 weeks after transplanted. Apoptosis was examined by tunnel assay and expression Brn3b (Brn3b = RGCs marker) by immunohistochemical analysis of the retina. Results showed that transplantation of hypoxic preconditioning BM-MSCs in acute glaucoma models resulted in a significant apoptosis decreasing (p < 0.05) and an significant increasing in RGCs (p < 0.05), as well as enhanced morphologic and functional benefits of stem cell therapy versus normoxic BM-MSCs transplantation. Conclusions: Hypoxic preconditioning enhances the capacity of BM-MSCs transplantation to improve neuroprotective effects of RGCs in Acute Glaucoma Models.

Item Type: Article
Uncontrolled Keywords: Hypoxic Preconditioning, Transplantation, Bone Marrow-Mesenchymal Stem Cells, BM-MSCs,Glaucoma, Neuroprotective
Subjects: Medicine
Divisions: Journal Publication
Depositing User: F.X. Hadi
Date Deposited: 20 Feb 2019 09:02
Last Modified: 31 Jan 2020 04:49
URI: http://repository.wima.ac.id/id/eprint/18099

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