Water Fraction Of Sambiloto (Andrographis Paniculata Nees) Ethanol Extract Efficacy In Inducing The Number Of Macrophage, Neutrophil, And The Level Of TNF-α On Wistar Rats

Tamayanti, Wahyu Dewi and Kristanti, Lidwina Tri and Kurniawan, Hendra and Ervina, Martha and Hadisoewignyo, Lannie and Soegianto, Lisa and Widharna, Ratna Megawati (2013) Water Fraction Of Sambiloto (Andrographis Paniculata Nees) Ethanol Extract Efficacy In Inducing The Number Of Macrophage, Neutrophil, And The Level Of TNF-α On Wistar Rats. In: International Conference On Central Management Of Central Cytotoxic Reconstitution, May 25th 2013, Yogyakarta.

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Abstract

Background. The study of the effect of water fraction of Sambiloto (Andrographis paniculata Nees) ethanol extract on the number of macrophage, neutrophil, and levet of TNF-α in the body fluid of male Wistar rats after Staphylococcus aureus induction. Methods. Animals were divided into 3 groups, namely: negative control (NC), treatment (T), and positive control (PC) group. The NC, T and PC groups were administered by 0,9% NaCL, water fraction of Sambiloto ethanol extract of 40mg/200g BW dose, and ibuprofen of 7.2 mg/200g BW dose, respectively. The treatment was performed for 7 consecutive days. The number of macrophages, neutrophils, was identifed using light microscope whereas the TNF-α. level was identified using ELISA. Outcome Measured. The number of macrophage, neutrophil, and level of TNF-α in the body fluid of male Wistar rats after Staphylococcus aureus induction. Results. The number of macrophages in the KC, the T, and the PC groups were 2.33 ± 1,58; 3.80 ± 4.06; and 2.67 ± 1.55 cells, respectively. The number of neutrophils were 0.83 ± 0.33; 0.58 ± 0.32; and 1.33 ± 0.38 cells respective to the KN, T, and PC groups. The level of TNF-α were I7.78 ± 11.67; 23.48 ± 15.95; and 27.90 ± 30.54 pg/ml respective to the KN, the T, and PC groups, Conclusion. This study indicated that water fraction of Sambiloto ethano! extract able to increase the number of macrophage and TNF-α level yet decrease the number of neutrophil in the body fluids of male Wistar rats after Staphylococcus aureus induction.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: macrophages, neutrophils, TNF-α, Herba Sambiloto, ibuprofen
Subjects: Pharmacy
Divisions: Proceeding > Faculty of Pharmacy
Depositing User: Josefine Hira Eksi
Date Deposited: 30 Jun 2016 06:30
Last Modified: 19 Jul 2016 03:00
URI: http://repository.wima.ac.id/id/eprint/6830

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