Optimizing Streptozotocin Dose for Inducing Type 1 Diabetes Mellitus in Male Wistar Rats.

Soeliono, Ivonne, Hadisoewignyo, Lannie, Kristiani, Dessy, Chang, Giovanni and Irawaty, Wenny (2018) Optimizing Streptozotocin Dose for Inducing Type 1 Diabetes Mellitus in Male Wistar Rats. In: Proceeding 1st International Conference on Pharmacy Science and Practice., 6-7 Desember 2018, STIFAR Riau, ISBN: 978-602-50854-1-3.

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Abstract

The preclinical antidiabetic study generally uses animal models with cytotoxic agent induction, e.g., streptozotocin (STZ). However, a different STZ dose (35-80 mg/kg) and routes of administration (intraperitoneally or intravenously) were mentioned before. This study has the aim to determine the optimal STZ intravenous dose for diabetes induction in rats. Sixty male Wistar rats (150-200 g) were undergone oral glucose tolerance test to examine their glucose homeostasis. A week later all rats were fasted for 8 hours and divided into six groups: STZ 40, 50, 60, 70, 80 mg/kg and control. Fasting blood glucose (FBG) level was measured on day 3, 7, 14 and 21 after STZ administration and was analyzed using a Kruskal-Wallis test. At day 21 all rats were sacrificed, and their pancreas was taken for histopathology examination. STZ given at 40,50,60, 70, 80 mg/kg resulted in death of 0%, 10%, 60%, 80%, 90% rats from each group. Three days after STZ administration there was a significant FBG increase in all STZ dose groups, except in 40 mg/kg group (p < 0.05). On the 7th day, the increasing FBG were persisted in 50, 60 and 70 mg/kg groups. On the 14th and 21st day, all rats FBG have decreased to <200 mg/dL. Histology examination revealed that a higher dose of STZ has resulted in lower Langerhans island number and diameter. The optimal dose of STZ to induce diabetic in male Wistar rats is 50 mg/kg. At this dose, hyperglycemia was persistent for seven days, and the mortality was minimal.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: optimal dose, streptozotocin, diabetes, Wistar rats.
Subjects: Pharmacy
Divisions: Proceeding
Depositing User: F.X. Hadi
Date Deposited: 01 Feb 2021 06:03
Last Modified: 08 May 2023 06:35
URI: http://repository.ukwms.ac.id/id/eprint/25087

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